Technical Data

H6098-13B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG2a
Clone Number
8.L.125B
Grade
Highly Purified
Applications
FC IF
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-HLA Class 1 Antigen A2,A28 (FITC)

Antibodies can also be used for the detection of HLA antigens on the lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue. The HLA Class 1 conjugated monoclonal antibodies can be used for the detection of specific cell surface antigens.

Applications
Suitable for use in Flow Cytometry. Other potential applications include Cell Typing, Tissue Staining and Chimerism Studies.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
FITC/Protein Ratio
4.0-8.0
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.  Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Ion Exchange chromatography.
Specificity
Recognizes the human HLA Class 1 Antigen A2,A28.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. R. Pei et al., Tissue Antigen 41:41:200-203 (1993). 3. R.. Pei, G.Woo and J.H. Lee, Visuals of the clinical histocompatibility workshop, Paul I. Terasaki, Ed., pp.73-74 (1995). 4. R. Pei, T. Chen, J. Orpilla, and J.H. Lee., Tissue Antigens 50:197-201 (1997).
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net