Technical Data

H6098-45B
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG2b
Clone Number
0.L.3
Grade
Highly Purified
Applications
FC IF
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-HLA Class 1 Antigen B8 (FITC)

Antibodies can also be used for the detection of HLA antigens on the lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue. The HLA Class 1 conjugated monoclonal antibodies can be used for the detection of specific cell surface antigens.

Applications
Suitable for use in Flow Cytometry. Other potential applications include Cell Typing, Tissue Staining and Chimerism Studies. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cells with B8 antigens.
Form
Supplied as a liquid in PBS, 0.05% sodium azide, 0.5% BSA. Labeled with Fluorescein isothiocyanate (FITC). FITC/Protein Ratio: 4-8.
Purity
Purified by Ion Exchange chromatography (≥98%).
Specificity
Recognizes human HLA Class 1 Antigen B8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50:197-201 (1997).
USBio References
No references available
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