Rabbit Anti-HLA Class 1 Antigen G (HLA Cass I Histocompatibility Antigen alpha Chain G, HLA G Antigen, HLA G, HLAG, HLA-G, HLA-6.0, Major Histocompatibility Complex Class I G, MHC-G)
B2 microglobulin, DADB-15K14.8, HLA 6.0, HLA class I histocompatibility antigen alpha chain G, HLA class I molecule, HLA G, HLA G antigen, HLA G3, HLA-G, HLA-G histocompatibility antigen, class I, HLA60,, HLAG_Human, Major histocompatibility complex class I G, MHC class I antigen, MHC class I antigen G, T-cell A locus, TCA
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45kD and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Immunohistochemistry (FFPE, Leica): 1:500. Requires HIER with EDTA buffer, pH 9.0 Indirect Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 62-89 in the central region of human HLA Class 1 Antigen G.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human HLA Class 1 Antigen G.
