Mouse Anti-HLA Class 2 Antigen DR (FITC)
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 -1:200. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Immunohistochemistry: Frozen. Not tested on paraffin tissues.
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents
Flow Cytometry: F(ab')2 Rabbit anti-mouse IgG labled with FITC or RPE Immunohistochemistry: F(ab')2 Rabbit anti-mouse IgG labled with HRP
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
HLA Class 2 Antigen DR
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes the human HLA-DR cell surface antigen. Recognizes a determinant that is dependent upon the association of both the a and b chains of HLA-DR.
