hnRNP Q/R

Clone Type

Polyclonal

Host

Rabbit

Source

Human

Swiss Prot

O60506, O43390

Isotype

IgG

Grade

Affinity Purified

Applications

IP WB

Crossreactivity

Gene ID

10492, 10236

Shipping Temp

Blue Ice

Storage Temp

-20°C

Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis and mRNA transport from nucleus to cytoplasm (1-3). These two proteins are encoded by different gene but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AUrich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulate RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (11).

Applications

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution

Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of human hnRNP Q.

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Purity

Purified by Protein A and peptide affinity chromatography.

Specificity

Recognizes endogenous levels of total hnRNP Q and hnRNP R.

References

(1) Dreyfuss, G. et al. (1993) Annu Rev Biochem 62, 289-321. (2) Burd, C.G. and Dreyfuss, G. (1994) Science 265, 615-21. (3) Krecic, A.M. and Swanson, M.S. (1999) Curr Opin Cell Biol 11, 363-71.|(4) Passos, D.O. et al. (2006) Biochem Biophys Res Commun 346, 517-25. (5) Moraes, K.C. et al. (2003) Biol Chem 384, 25-37. (6) Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52. (7) Rossoll, W. et al. (2002) Hum Mol Genet 11, 93-105. (8) Liu, H.M. et al. (2009) Virology 386, 249-56. (9) Hassfeld, W. et al. (1998) Nucleic Acids Res 26, 439-45. (10) Carty, S.M. and Greenleaf, A.L. (2002) Mol Cell Proteomics 1, 598-610. (11) Mourelatos, Z. et al. (2001) EMBO J 20, 5443-52.

USBio References

No references available

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