Mouse Anti-Huntingtin Protein, aa2703-2911 (Huntington Disease)
Huntington's disease (HD) is a neurodegenerative disorder caused by an expanding polyglutamine repeat in the huntingtin gene. HD is a mid-life onset autosomal dominant neurodegeneative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.
Applications
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen. Increased cytoplasmic staining, relative to nuclear, has been reported using formaldehyde as a fixative compared with acetone/methanol. Optimal dilutions to be determined by the researcher.
Recommended Positive Control Tissue
Brain
Recommended Secondary Antibody
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa2703-2911 of human Huntingtin Protein.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes an epitope corresponding to the human HDC region (aa2703-2911) of the Huntingtin protein. Detects a 350kD band on Western Blots but also detects smaller degradation products of Huntingtin. Recognizes both denatured and native Huntingtin in human brain. The combined use of H7960-01, H7960 and H7960-01A demonstrate that huntingtin is enriched in neuronal cells in the brain. Species Crossreactivity: rabbit and mouse.
