Goat Anti-Hydroxyoctadecadienoic acid (HODE) 9(±)
Linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, can be metabolized by cyclooxygenase, lipoxygenase and P450 enzymes. The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(R)-HODE, 9(S)-HODE and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites.
These compounds exhibit interesting biologic activities, including the regulation of platelet function, maintenance of vascular thromboresistance and transduction of the cellular responses to certain growth factors. HODE derivatives may also influence certain pathological states including, psoriasis, the development of atherosclerosis and the development of cancer.
Applications
Suitable for use in RIA and ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 2ug/well RIA: 2ug/well Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
9(S)-HODE; 9(R)-HODE.
Form
Supplied as a liquid in PBS.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes 9(±)-HODE. Crossreactivity: 9(±)-HODE: 100.0%, 9(S)-HODE: 100.0%, 9(R)-HODE: 100.0%, 13(S)-HODE: 1.2%, 13(R)-HODE: 1.2%, 9-oxo-ODE: 1.2%, 13-oxo-ODE: 2.4%, 11(S)-HETE: 0.0%, 15(S)-HETE: 0.0%, Linoleic Acid: 0.0%.
