MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in FLISA, Immunohistochemistry, Western Blot and Immunodiffusion. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: frozen sections Western Blot: Reducing conditions Optimal working dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified canine IgA
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by affinity chromatography.
Specificity
Recognizes canine IgA and shows no crossreactivity with other canine immunoglobulin classes in immunoelectrophoresis. Has been shown to crossreact with IgA from other canids and with mustelids. Species crossreactivity: wolf, ferret.