Mouse Anti-IgA, Secretory Component (FITC)
IgA is the most abundant immunoglobulin in body fluids and the second most abundant immunoglobulin in plasma, found at a concentration of 0.4 to 2.2mg/ml. It plays a very important role in the first specific defense against natural infection. Secretory IgA differs from serum IgA in that it contains two additional peptides: the secretory component and the J chain.
Applications
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen and Paraffin sections Optimal working dilutions to be determined by researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from ascites
Specificity
Recognizes human secretory-piece and secretory IgA. No reaction against myeloma IgA (native or reduced and alkylated). Negative against normal human IgM, myeloma IgM, normal human IgG; free kappa or gamma chains and J-chain. Typical pattern on frozen and paraffin embedded sections of intestinal and breast tissue.
