Rat Anti-IgE, Heavy Chain (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Fusion Partners: Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Applications
FLISA: 5ug/ml as capture antibody Optimal working dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified IgE from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the epsilon heavy chain of mouse immunoglobulin. Does not react with rat.
