Goat Anti-IgG, H&L, X-Adsorbed (Cy2)
Applications
Suitable for use in FLISA and Immunofluorescence. Other applications not tested.
Recommended Dilution
IF Microscopy: 1:100-1:1000 Optimal dilutions to be determined by the researcher.
Absorption Wavelength
489 nm
Emission Wavelength
505nm
Fluorochrome/Protein Ratio
3.1 moles Cy2 per mole of Goat IgG
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse IgG, whole molecule
Form
Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide, and 10mg/ml BSA (IgG and Protease free). Reconstitute with 1ml sterile dH2O. Labeled with Cy2 (Cyanine 2-OSu, MW 897D).
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes Mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG and mouse serum. No reaction was observed against bovine, chicken, goat, guinea pig, hamster, equine, human, rat, rabbit and sheep serum proteins.
