Rabbit Anti-IgG (H&L) (AP)
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:50,000-1:5000 Western Blot: 1:50,000-1:5000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Immunogen
Purified rat IgG H&L
Form
Supplied as a liquid in 30mM triethanolamine, pH 7.2, 5mM magnesium chloride, 0.1mM zinc chloride, 1% BSA, protease/IgG free, 0.05% sodium azide. Labeled with Alkaline Phosphatase (AP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat IgG H&L.
