Rabbit Anti-IgG, Fc (Texas Red)
Applications
Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.
Recommended Dilution
Optimal dilution determined by the researcher.
Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.0 moles Texas Red per mole of Goat IgG
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Rat IgG F(c) fragment
Form
Supplied as a liquid in PBS, pH 7.2, 10 mg/ml BSA (IgG and Protease free), 0.01% Sodium Azide, 40% glycerol. Label: Texas Red Sulfonyl Chloride (Molecular Weight 625 daltons)
Purity
Purified by immunoaffinity chromatography.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab’)2.
