I1904-75L1-ML490
Clone Type
MonoclonalHost
RatSource
MouseConjugate
MaxLight™490Isotype
IgG1Clone Number
3H2301 (LO-MG1-13)Grade
Affinity PurifiedApplications
FLISACrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rat Anti-IgG1 (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in FLISA. Other applications have not been tested.
Recommended Dilutions
Optimal working dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified mouse IgG1 from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the gamma 1 heavy chain of mouse immunoglobulin. No crossreactivity with other immunoglobulin classes or subclasses. Does not crossreact with human.