I1904-75M-ML650
Clone Type
MonoclonalHost
MouseSource
RatConjugate
MaxLight™650Isotype
IgG1Clone Number
3H2316 (marg1-2)Grade
Affinity PurifiedApplications
FLISACrossreactivity
RtShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-IgG1 (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
Optimal working dilutions to be determined by researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat IR27 IgG1 myeloma protein
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the heavy chain of rat IgG1. No crossreactivity is observed with other rat immunoglobulin classes or subclasses.
