Technical Data

I1904-75M
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Isotype
IgG1
Clone Number
3H2316 (marg1-2)
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-IgG1
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 3-5ug/ml Optimal working dilutions to be determined by researcher.
Recommended Pair
Suitable for use as the capture or solid phase antibody. Matched to K0100-01B as the detection or conjugate (label) antibody with purified rat IgG1 as the antigen.
Hybridoma
Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Rat IR27 IgG1 myeloma protein
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the heavy chain of rat IgG1. No crossreactivity is observed with other rat immunoglobulin classes or subclasses.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pelegri, C., et al., Clin. Exp. Immunol. 125: 470-477 (2001). 2. Sato, K., et al., J. Immunol. 166: 4185-4194 (2001). 3. Bezie S., et al., PLoS One. 10 (3): e0119686 (2015). 4. Bezie S., et al., Journal of Immunology 195(12):5805-15 (2015).
USBio References
No references available
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