Technical Data

I1904-80F-FITC
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1,k
Clone Number
3H2343 (HP6014)
Grade
Purified
Applications
FLISA IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Mouse Anti-IgG2 (FITC)
Applications
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human IgG2
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified
Specificity
Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Jefferis, R. et al. (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG sub-classes. Results of an IUIS/WHO collaborative study. Immunology Letters. 10: 223-252.2. Hussain, R. et al. (1986). 2. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of human IgG. J. Immunol. Meth. 93: 89-96. 3. Jefferis, R. et al . (1988). Immunology. 63: 171.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net