Mouse Anti-IgG2 (FITC)
Applications
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human IgG2
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity
Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.