Technical Data

I1904-80F-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
3H2343 (HP6014)
Grade
Purified
Applications
FLISA IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-IgG2 (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human IgG2
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified
Specificity
Recognizes human IgG2 at an epitope localized to the Fab region. Will not cross-react with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD as tested by hemagglutination assays.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Jefferis, R. et al. (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG sub-classes. Results of an IUIS/WHO collaborative study. Immunology Letters. 10: 223-252.2. Hussain, R. et al. (1986). 2. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of human IgG. J. Immunol. Meth. 93: 89-96. 3. Jefferis, R. et al . (1988). Immunology. 63: 171.
USBio References
No references available
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