Rat Anti-IgG2a (HRP)
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 5ug/ml as coating antibody Optimal dilutions to be determined by the researcher.
Recommeded Pair
Coating: I1904-80I Detection: I1904-83G1
Hybridoma
Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified IgG2a from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse gamma 2a heavy chain.