Technical Data

I1904-80I-HRP
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
HRP
Isotype
IgG1
Clone Number
3H2348 (LO-MG2a-9)
Grade
Affinity Purified
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Rat Anti-IgG2a (HRP)
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 5ug/ml as coating antibody Optimal dilutions to be determined by the researcher.
Avidity
2.5x10e9Me-1
Recommeded Pair
Coating: I1904-80I Detection: I1904-83G1
Hybridoma
Spleen cells from immunized LOU/c rat were fused with cells of the IR983F rat myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
Purified IgG2a from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse gamma 2a heavy chain.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lahat, G. et al. (2010) Vimentin is a novel anti-cancer therapeutic target; insights from in vitro and in vivo mice xenograft studies. PLoS One. 5(4):e10105. 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 185:7527-36 3. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein. Vaccine. 24: 4102-10. 4. Hajek, A.R. et al. (2008) 12/15-Lipoxygenase deficiency protects mice from allergic airways inflammation and increases secretory IgA levels. J Allergy Clin Immunol. 122: 633-9.e3.
USBio References
No references available
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