Technical Data

I1904-82P-PE
Clone Type
Monoclonal
Host
Mouse
Source
Rat
Conjugate
PE
Isotype
IgG1
Clone Number
3H2362 (MARG2a-1)
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-IgG2a, Heavy Chain (PE)
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
Optimal working dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the mouse PAI-O myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Rat IgG2a (polyclonal)
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography.
Specificity
Recognizes the rat IgG2a heavy chain. Does not crossreact with other rat immunoglobulin classes or subclasses.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bazin, H., Beckers, A. & Querinjean, P., Eur. J. Immunol. 4: 44-48 (1974). 2. Kohler, G. & Milstein, C., Nature 256: 495-497 (1975). 3. Schulman, M., Wilde, C.D. & Kohler, G., Nature 276: 269-270 (1978). 4. Bazin, H., Beckers, A., Deckers, C. & Moriame, M., J. Natl. Cancer Institute 51: 1359-1361 (1973). 5. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Platteeu, B. & Urbain, J., J. Immunol. 121: 2077-2082 (1978).
USBio References
No references available
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