Technical Data

I1904-83A
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG1
Clone Number
3H2371 (LO-MG2a-7)
Grade
Affinity Purified
Applications
E
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-IgG2a, Heavy Chain
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 5ug/ml as coating antibody. Optimal working dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified IgG from BALB/c mice.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the mouse gamma 2a immunoglobulin heavy chain. Recognizes an allotypic determinant upon mouse IgG2a. Reacts with the IgHIa allotype (as expressed in BALB/c mice). Does not react with the IgHIb allotype (as expressed in C57/BL mice).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Jarman, E., et al., Immunology 112: 631-642 (2004). 2. Bazin, H., (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids. 615-618. Pergamon Press, Oxford and New York. 3. Bazin, H., et al., J. Immunol. Methods 66: 261-269. (1984) 4. Bazin, H., et al., J. Immunol. Methods. 71: 9-16 (1984). 5. Querinjean, P., et al., Eur. J. Biochem. 31: 354-359 (1972). 6. Nagao, K., et al., Am. J. Respir. Cell. Mol. Biol. 29: 314-320 (2003). 7. Ramos, J.D.A., et al., Philippine Science Letters 2: 38-48 (2009). 8. Ormstad, H., et al., Toxicology 188: 309-318 (2003). 9. Ormstad, H., et al., J. Toxicol. Environ. Health A 61: 55-67 (2000). 10. Hall, G., et al., Vaccine 21: 549-561 (2003). 11. Tan, L.K., et al., Vaccine 24: 5762-5771 (2006). 12. Instanes, C. & Hetland, G., Toxicology 204: 13-21 (2004). 13. Lieden, A., et al., Allergy 64: 304-311 (2009). 14. Wolfowicz, C.B., et al., Vaccine 21: 1195-1204 (2003).
USBio References
No references available
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