Technical Data

I1904-84P-ML650
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
MaxLight™650
Isotype
IgG1
Clone Number
3H2407 (LO-MG2b-1)
Grade
Affinity Purified
Applications
FLISA
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rat Anti-IgG2b (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Rat IR983F myeloma cells with spleen cells from immunized LOU/c rats.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. 
Note: Applications are based on unconjugated antibody.
Immunogen
Polyclonal IgG from BALB/c mice.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by affinity chromatography from tissue cuture supernatant.
Specificity
Recognizes mouse IgG2b, binding to the gamma 2b heavy chain.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. J Immunol. 185:7527-36.
USBio References
No references available
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