I1904-89B1-ML650
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™650Isotype
IgG1Clone Number
3H2426 (HP6025)Grade
PurifiedApplications
FLISA IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-IgG4 (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen and paraffin sections. Optimal dilutions to be determined by the researcher.
Hybridoma
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human IgG4.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Specificity
Recognizes the heavy chain of human IgG4 at an epitope in the Fc region. Does not crossreact with IgG1, IgG2, IgG3, IgM or IgA. Species Crossreactivity: chimpanzee
