Suitable for use in Flow Cytometry, Immunohistochemistry, FLISA and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human IgM
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes human IgM, binding to the mu heavy chain. Does not cross-react with other immunoglobulin classes.
References
1. Cragg, M.S. et al. (1999). Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells. Br. J. Cancer. 79: 850-7.