Suitable for use in FLISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified canine IgM
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Purity
Purified by affinity chromatography
Specificity
Recognizes canine IgM and shows no crossreactivity with other canine immunoglobulin classes in immunoelectrophoresis and ELISA. May crossreact with IgM from other species.
References
1. Peters, I. R. et al.. Clin. Diag. lab. immunol. 11; 841-848.(2004).