Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified Peking duck yolk IgY
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. Species Crossreactivity: goose. Does not react with chicken.
References
1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748.|2. Schneider, K. et al. (2004) Chicken BAFF – a highly conserved cytokine that mediates B cell survival. Int. Immunol. 16: 139-148.