Mouse Anti-IgY, Heavy Chain
The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab’)2 fragment of IgY.
Applications
Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul. Western Blot: Non-reducing Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified Peking duck yolk IgY
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes both the IgY and IgY(-Fc) Peking duck heavy chain molecules. Species Crossreactivity: goose. Recognizes ~118kD and 178-200kD in pekin cell lysates under non-reducing conditions. Recognizes IgY heavy chains in Mallard duck, goose and swan.
