Rabbit Anti-IkB beta, CT (Not for Export EU)
NFkB exists in the cytoplasm as a complex with a 38kDa inhibitor protein, IkB (MAD-3). Activation of NFkB by different stimuli, including TNFa and PMA, requires the dissociation of the inhibitor protein. Dissociation is regulated by the phosphorylation of IkB on serine residues 32 and 36 which targets IkB for ubiquinitation and proteasome mediated degradation. The NFkB complex then translocates to the nucleus and activates transcription of responsive genes.
Application(s)
Suitable for Immunoprecipitation, Western Blot, ELISA.
Recommended Dilution
ELISA: 1:1000-1:5000 Western Blot: 1:500-1000 (Peroxidase conjugated goat anti-rabbit IgG (H&L). Anti-IkB beta is suitable for the detection by immunoblot of human, mouse and rat IkB beta.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
IkB beta peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH. AA Sequence: RQP PSP ASK PLP DDP NPA
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity
Purified by delipidation and defibrination.
Specificity
Recognizes 45kD human IkBb. May react non-specifically with other proteins. Species crossreactivity: mouse and rat.
