Rabbit Anti-IkB zeta (IkappaB zeta, IKBZ, FLJ30225, FLJ34463, INAP, Molecule Possessing Ankyrin Repeats Induced by Lipopolysaccharide, MAIL Protein, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B cells Inhibitor zeta, NFKBIZ)
IkappaB zeta (also known as MAIL and INAP) is an ankyrin-repeat-containing nuclear protein that is highly homologous to the IkappaB family member Bcl-3. Transcription of IkappaBzeta is upregulated by stimulation with TLR ligands, IL-1 and IL-6 in cultured B-lymphocytes and monocytes/macrophages, but only faintly so in T-lymphocytes, fibroblasts, and endothelial cells. IkappaB-zeta preferentially associates with the NF-kappaB subunit p50 rather than p65 and recombinant IkappaB-zeta proteins inhibit the DNA binding of the p65/p50 heterodimer and the p50/p50 homodimer.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptides corresponding to aa684-699 RKGADPSTRNLENEQP and aa285-298, YQYSPQTQNMQQPP of mouse IkBz protein (NP_085115.1). Species Sequence Homology: human, rat, bovine and primate; 100%, chicken; 93%
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse IkB zeta.
