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I5005-02F Rabbit Anti-ILK, CT (Integrin Linked Kinase, Integrin-linked Protein Kinase, 59kD Serine/threonine Protein Kinase, DKFZp686F1765, ILK 1, ILK1, ILK-1, ILK 2, ILK2, ILK-2, p59, p59ILK)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
O55222
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. ILK encodes a predicted 451aa protein, with an apparent molecular weight of 59kD. The ILK protein is a serine/threonine protein kinase with 4 ankyrin-like repeats. ILK regulates integrin-mediated signal transduction.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human ILK1 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human ILK1.
References
1.Li, Y., et al., J. Clin. Invest. 112(4):503-516 (2003). 2.Troussard, A.A., et al., J. Biol. Chem. 278(25):22374-22378 (2003). 3.Marotta, A., et al., Br. J. Cancer 88(11):1755-1762 (2003). 4.Cordes, N., et al., Br. J. Cancer 88(9):1470-1479 (2003). 5.Fukuda, T., et al., J. Cell Biol. 160(7):1001-1008 (2003).
USBio References
No references available
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