Mouse Anti-Indoleamine 2,3-dioxygenase (Indoleamine-pyrrole 2,3-dioxygenase, IDO, INDO, Indoleamine 2,3-dioxygenase 1, IDO1, IDO-1)
EC=1.13.11.52, CD107B
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumors and blocking the IDO pathway may be a potential target for immunotherapy.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended. Western Blot: Recognizes a 45kD band in IFN-gamma treated cell lines. Recognizes a slighty lower MW band in mouse IDO compared to its human counterpart. Optimal dilutions to be determined by the researcher.
Positive Control
IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
Storage and Stability
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide corresponding to aa78-184 of human IDO fused to GST. Species sequence homology: mouse and rat
Form
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Specificity
Recognizes human Indoleamine-pyrrole 2,3 - dioxygenase (IDO).
