Technical Data

I7649-40C
Clone Type
Monoclonal
Host
Mouse
Isotype
IgG
Clone Number
7G45 (4F1F2)
Grade
Affinity Purified
Applications
E
Accession #
AAT76166
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Influenza A, Avian, H5N1, Hemagglutinin (HA) (Bird Flu)

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). The more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low (4). HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5). While efforts were made to use relatively conserved regions of the viral sequence as the antigen, the influenza virus genome has drifted somewhat from what was first reported. However, this antibody was able to recognize peptides derrived from viruses from Indonesian human patients infected in 2007.

Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1ug/ml detects 10ng of free peptide. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 14aa near the middle of the Hemagglutinin protein.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Hemagglutinin protein from the H5N1 strain of avian influenza (IN).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Thompson, W.W., Shay, D.K., Weintraub, et al., JAMA 289: 179-186 (2003). 2. Alexander, D.J., Vet. Microbiol. 74: 3-13 (2000). 3. Shortridge, K.F., Zhou, N.N., Guan, Y., et al., Virol. 252: 331-342 (1998). 4. Buxton Bridges, C., Katz, J.M., Seto, W.H., et al., J. Inf. Dis. 181: 344-348 (2000). 5. Iwatsuki-Horimoto, K., Kanazawa, R., Sugii, S., et al., J. Gen. Virol. 85: 1001-1005 (2004).
USBio References
No references available
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