I7655-65C
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E WBCrossreactivity
Hu MoAccession #
NP_002211Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Inositol 1,4,5-trisphosphate 3-kinase A, CT (Inositol-trisphosphate 3-kinase A, InsP 3-kinase A, IP3 3-kinase A, IP3K A, IP3KA, IP3-3KA, ITPKA)
EC=2.7.1.127
ITPKA regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of the inositol 1,4,5-trisphosphate 3-kinase is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. It is also a substrate for the cyclic AMP-dependent protein kinase, calcium/calmodulin- dependent protein kinase II, and protein kinase C in vitro. ITPKA and ITPKB are 68% identical in the C-terminus region.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human ITPKA (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human ITPKA. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1.Gonzalez, B., et al., Mol. Cell 15(5):689-701 (2004). 2.Mishra, J., et al., Biophys. J. 83(3):1298-1316 (2002). 3.Schell, M.J., et al., J. Biol. Chem. 276(40):37537-37546 (2001). 4.Communi, D., et al., EMBO J. 16(8):1943-1952 (1997). 5.Woodring, P.J., et al., J. Biol. Chem. 272(48):30447-30454 (1997).USBio References
No references available