Mouse Anti-Insulin
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.
Applications
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Matched Pairs
Capture: I7656-06 Detection: I7656-07
Affinity Constant
1.5 x 10e8 M-1
Hydridoma
X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human insulin
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Glycerol free.
Purity
Purified by Protein A affinity chromotography (>90% by electrophoresis).
Specificity
Recognizes human insulin. Does not crossreact with free C-peptide. Species Crossreactivity: human proinsulin, bovine (30%) and porcine insulin.
