Rabbit Anti-Insulin-Like Growth Factor II (IGF2, IGF II, IGF-II, C11orf43, FLJ22066, FLJ44734, INSIGF, PP1446, pp9974, Somatomedin A) (MaxLight 550)
Insulin-like Growth Factor 2
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Insulinlike Growth Factor II, a 67aa mitogenic polypeptide and member of the Insulin gene family, synthesized by various foetal tissues during prenatal development, under the influence of placental lactogen. IGFII is a growth factor which exhibits both paracrine and autocrine properties and signals by binding to the type 1 and type 2 insulinlike growth factor receptors (type 1 IGFR and type 2 IGFR), and also to the insulin receptor (IR). The availability of IGFII is dependent upon its attachment to, and release from, various IGF binding proteins (IGFBPs), a relationship which has been investigated in breast cancer research (2).
Applications
Suitable for use in FLISA, Functional Assays, and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.5ug/ml Western Blot: 0.1-0.2ug/ml Functional Assays: 3.1-4.6ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant human IGF-II.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IGF-II.
