Rabbit Anti-Insulin-like Growth Factor Binding Protein 7 (IGF Binding Protein 7, IGFBP7, IGFBP-7, IGFBP-7v, IBP-7, FSTL2, IGFBPRP1, IGFBP-rP1, MAC25 Protein, MAC25, Prostacyclin-stimulating Factor, PGI2-stimulating Factor, PSF, Tumor-derived Adhesion Factor, TAF) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Insulinlike growth factor binding protein7, also known as mac25, a 256 aa secreted protein and member of a growing family of IGFBPs which regulate the biological activities and distribution of the insulinlike growth factors (IGFs), through binding to IGFI and IGFII. IGFBP7 is widely expressed throughout normal tissues, but is also expressed in many tumor types at an increased or reduced level, and acts as a suppressor of breast and prostate cancer cell lines. IGFBP7 inhibits IGFinduced differentiation of skeletal muscle (myogenesis) and has implications in bone formation, by its possible affects on the actions of parathyroid hormone (PTH) and cortisol.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human IGFBP7, expressed in E coli.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IGFBP-7.
