Sheep Anti-Insulin-Like Growth Factor Binding Protein-Related Protein 10 (IGFBP-rP10, Kazal-type Serine Protease Inhibitor Domain-containing Protein 1, Bone and Odontoblast-expressed Protein 1, Insulin-like Growth Factor-binding-related Protein 10, IGFBP-rP10, IGFBP-related Protein 10, Insulin-like Growth Factor-binding-related Protein 4, Kazald1, Bono1) Biotin
IGFBP-rP10 (IGF binding protein-related protein 10; also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37kD (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274 aa in length. It contains an IGF binding domain (aa49-124), a follistatin-like serine protease inhibitor region (aa125-168) and a C2-type Ig-like domain (aa172-269). There is one splice variant that shows a 70 aa substitution for aa184-304. Mature human IGFBP-rP10 is 87% aa identical to mouse IGFBP-rP10.
Applications
Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, NS0-derived, recombinant human IGFBP-rP10 (aa31-304; Accession # Q96I82).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IGFBP-rP10. Species sequence homology: mouse (87%)
