I7661-23T-FITC
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
FITCIsotype
IgGGrade
Affinity PurifiedApplications
FLISA IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Rabbit Anti-INSR, NT (Insulin Receptor, IR, CD220) (FITC)
INSR is a receptor that binds insulin and has a tyrosine-protein kinase activity. Autophosphorylation activates the kinase activity. This Type I mebrane protein is composed of a tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. Defects in INSR are the cause of insulin resistance of various forms, including mild insulin-resistant diabetes mellitus with acanthosis nigricans, minor physical abnormalities and sometimes polycystic ovaries. Insulin resistance associated with acanthosis nigricans, hirsutism and hyperandrogenism is referred to as insulin resistance type A. Defects in INSR are the cause of Rabson-Mendenhall syndrome, also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin-resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities.
Applications
Suitable for use in FLISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa21-52 in the N-terminal region of human INSR, UniProt Accession #P06213|Species Sequence Homology: mouse and rat
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human INSR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. George, S., et al., Endocrinology 144(2):631-637 (2003). 2. Longo, N., et al., Hum. Mol. Genet. 11(12):1465-1475 (2002). 3. Hamer, I., et al., Diabetologia 45(5):657-667 (2002). 4. Osawa, H., et al., Clin. Genet. 59(3):194-197 (2001). 5. Rique, S., et al., Clin. Genet. 57(1):67-69 (2000).USBio References
No references available