Mouse Anti-Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
CD29 (integrin Beta-1/gpIIa) is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. The CD29 is expressed at the cell surface exclusively as part of a heterodimer, in association with one of at least nine different integrin alpha subunits (alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alpha7, alpha8, and alphav). With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed on nearly all cell and tissue types.
Applications
Suitable for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human melanoma V+B2 cell line. Cellular Localization: Cell membrane
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Integrin, beta1. Reacts with non-reduced protein of 110kD and a reduced protein of 130kD. Species Crossreactivity: porcine
