Mouse Anti-Integrin, beta3 (ITGB3, Integrin, beta 3, platelet glycoprotein IIIa, antigen CD61, GP3A, GPIIIa)
Integrin, beta 3 (Platelet Glycoprotein IIIa, Antigen CD61), Platelet Fibrinogen Receptor beta Subunit
b3-Integrins contain receptors for vitronectin, fibrinogen, and Von Willebrands factor and, in some instances fibronectin. The b3-Integrin subunit is a common subunit protein for this integrin family. The aIIb-Integrin subunit is a specific marker for platelets and megakaryocytes and can be utilized for this purpose in various immunological techniques. Monoclonal antibodies against the b3-Integrin subunits and the aIIb-Integrin subunit can be used in immunoblotting, immunoprecipitation and immunostaining experiments.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: Frozen Optimal dilutions to be determined by the researcher.
Hybridoma
Myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partially purified platelet glycoprotein IIb/IIIa.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
Specificity
Recognizes human b3-Integrin, including the GPIIIa of platelets.
