Technical Data

I7662-10P
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP N WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Interferon Beta (IFN-beta, Ifnb1, Ifb, Ifnb)

interferon beta (IFN Beta), a 22kD cytokine with wide-ranging antiviral, antimicrobial and antitumor effects. Interferons also serve to activate macrophages, natural killer cells and the presentation of antigens to the immune system by the major histocompatibility complex. The production of interferon is induced in response to microbes and other cytokines. The interferons are widely used to treat a variety of diseases and IFNB in particular is used to treat relapsing-remitting multiple sclerosis.

Applications
Suitable for use in Neutralization, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5unit/ml of interferon is the quantity necessary to produce acytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This materialis prepared specifically for effective neutralization of Mu-IFN-beta.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant corresponding to mouse interferon beta expressed in E.coli.
Form
Supplied as a liquid in PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse interferon beta.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Asselin-Paturel et al. (J Exp. Med, 2005) used 32400 in combination with Rabbit mouse IFN-alpha (32100) to explore the role of Type I IFN in the migration and activation of dendritic cells. Kamath et al. (J. Immunol. 2005) used a similar cocktail to demonstrate that dendritic cell derived Type I IFN stimulates bystander T-cells. 2. Seimon et al. (P.N.A.S, 2006) used 32400 to examine the role of IFN-Beta in protecting LPS treated macrophages from apoptosis. Zheng et al. (J. Biol. Chem, 2006) used 32400 to examine the role of autocrine IFN-Beta in RANKL stimulated iNOS expression in macrophages.
USBio References
No references available
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