Rabbit Anti-Interferon beta (IFNb)
IFN beta, initially described as fibroblast interferon, is produced predominantly by fibroblasts and some epithelial cells. Type 1 interferons are produced primarily in response to viral infections as part of the host immune response and can also inhibit the development of tumors. IFN-beta is involved in the regulation of non-specific humoral immune responses and responses against viral infections.
Applications
Suitable for use in Western Blot, ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 4-10ug/ml detects a band of ~15kD in A20 cell lysates. Immunohistochemistry (Paraffin): 2.5ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0 is recommended. Immunofluorescence: 20ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to 17aa near the center of human Interferon beta. Eptitope: within aa110-160
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human interferon beta (IFN-beta), a 22kD member of the type I alpha/beta interferon family. Species Crossreactivity: mouse and rat
