Mouse Anti-Interferon gamma (IFNg)
The cytokine human interferon-gamma (IFN-gamma) is a glycosylated protein that exists as a homodimer of 34 kd in its biologically active form. IFN-gamma is expressed upon activation by TH1 cells CD8+ cells and NK cells (2). Secreted IFN-gamma is a potent inhibitor of in vitro hematopoiesis and appears to serve as an important regulator of lymphocyte and macrophage function by exerting antiproliferative effects (1).
Applications
Suitable for use in Flow Cytometry (intracellular). Other applications not tested.
Recommended Dilution Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant human IFN-gamma
Form
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate.
Purity
Purified by Protein A affinity chromatography.
Specificity
Binds to recombinant and cellular IFN-gamma in ELISA and Flow cytometry.
