Technical Data

I7662-16V
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
F3
Grade
Affinity Purified
Applications
E IP N
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Interferon gamma (IFNg)
Applications
Suitable for use in Functional Studies, ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Functional Studies: can be used for in vitro and in vivo inhibition of activity of IFN-gamma. For neutralization of biological activity dilutions have to be made according to the amount of Interferon to be inactivated. ELISA: 1:50 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Unit Definition
One neutralizing unit is defined as the amount of antibody sufficient for neutralizing one unit of mouse gamma Interferon antiviral activity as calibrated against NIH standard Gg02-901-533.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes and neutralizes both natural and recombinant mouse gamma Interferon. Its binding and neutralizing activity has been demonstrated in vitro and in vivo. F3 antibodies have been demonstrated to be able to inhibit inflammatory responses to bacterial lipopolysaccharides. These antibodies were furthermore shown to inhibit Shwartzman reactions and to protect NZB mice against spontaneous development of autoimmune disease. Does not react with rat or human gamma interferon.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Armstrong, JA et al; Semi-micro, dye-binding assay for rabbit interferon. J Appl Microbiol 1971, 21: 723. 2. van Tiel, FH et al; Detection of Semliki Forest virus in cell culture by use of an enzyme immunoassay with peroxidase-labeled monoclonal antibodies specific for glycoproteins E1 and E2. J Clin Microbiol 1984, 20: 387. 3. Dijkmans, R et al; Heterogeneity of Chinese hamster ovary cell-produced recombinant murine interferon-gamma. J Biol Chem 1987, 262: 2528. 4. Heremans, H et al; Regulation by interferons of the local inflammatory response to bacterial lipopolysaccharide. J Immunol 1987, 138 : 4175. 5. Billiau, A et al; anti-interferon-gamma antibody protects mice against the generalized Shwartzman reaction. Eur J Immunol 1987, 17 : 1851. 6. Jacob, CO et al; in vivo treatment of (NZB X NZW) F1 lupus-like nephritis with monoclonal antibody to gamma interferon. J Exp Med 1987, 166: 798. 7. Wolfs, T et al; In vivo expression of Toll-like receptor 2 and 4 by renal epithelial cells: IFN-gamma and TNF-alpha mediated up-regulation during inflammation. J immunol 2002, 168: 1286.
USBio References
No references available
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