Technical Data

I7663-25J1
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
Reg20
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Interleukin 1 Receptor Type I, p80 (IL-1RI)

The IL-1 system includes two agonists (IL-1alpha and IL-1beta), converting enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor.

Applications
Can be used for immunoassays, Western Blot, Immunoprecipitation and purification. Other applications not tested.
Recommended Dilution
Western Blot: 1:10 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes soluble mouse interleukin-1 receptor type I.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kojouharoff, G et al; Neutralization of tumour necrosis factor (TNF) but not of IL-1 reduces inflammation in chronic dextran sulphate sodium induced colitis in mice. Clin Exp Immunol 1997, 107: 353. 2. Stern, A et al; Purification to homogeneity and amino acid sequence analysis of a receptor protein for interleu- kin-1. Arch Biochem Biophys 1989, 274: 26.
USBio References
No references available
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