Technical Data

I7663-31A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
7G7/B6
Grade
Affinity Purified
Applications
IC
Crossreactivity
Hu
Accession #
NM_000417.1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
BSA Free
Mouse Anti-Interleukin 2 Receptor, alpha (IL-2Ra)
Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunocytochemistry: 5-10ug/ml detects the IL2 receptor on ice cold ethanol/acetic acid [95:5] fixed PHA-treated Jurkat T lymphocytes. Optimal dilutions to be determined by researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Whole cells expressing human IL-2 receptor
Form
Supplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human cell-surface and soluble IL-2 receptor at 55kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Tahilian, P.D., et al., J. Biol. Chem., 272: 7892-7898, 1997.
USBio References
No references available
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