Mouse Anti-Interleukin 3 Receptor alpha (IL-3RA, IL-3R alpha, IL-3R, IL3RAX, IL3RX, IL3RAY, IL3RY, CD123, MGC34174)
Interleukin 3 Receptor alpha chain
IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.
Applications
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry (Intrecellular): 1:10-1:1000. Use 1ug to label 1x10e6 cells in 100ul Western Blot: 1:100-1:2000. Detects a band at ~70kD Immunohistochemistry (Frozen): 1:10-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
IL3R transfected COS cells. Cellular Localization: Type I membrane protein
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Interleukin 3 Receptor alpha at ~70kD
