Technical Data

I8427-10D
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a,k
Clone Number
TRFK4
Grade
Purified
Applications
E IC IHC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rat Anti-Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Interleukin 5 is a T-cell-derived glycoprotein which stimulates eosinophil colony formation and is an eosinophil differentiation factor in humans and mice. It is also a growth and differentiation factor for mouse but not human B cells.

Applications
Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
ELISA: (as a detection antibody) 0.25ug/ml. Pairs with I8427-12C Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Semi-purified mouse T cell clone supernatant
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity
Purified
Specificity
Recognizes mouse Interleukin-5 (IL-5).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Schumacher, J.H., A. O’garra. B. Schrader, A. Van Kimmenade, M.W. Bond, T.R. Mosmann, and R.L. Coffman. 1988. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 ELISA. J. Immunol. 141:1576-1581.|2. Abrams, J.S., M.-G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti-cytokine monoclonal antibody development: Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127:5-24.|3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit 6.20.|4. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and sera. J. Immunol. Meth. 166:201-214.
USBio References
No references available
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