Rabbit Anti-Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Applications
Suitable for use in FLISA, Western Blot, Immunohistochemistry and Neutralization. Other applications have not been tested.
Recommended Dilutions
Western Blot: Reducing or non-reducing conditions. Immunohistochemistry (FFPE): Formalin fixed and paraffin embedded tissues human pancreas adenocacinoma tissue. Heat induced antigen retrieval with sodium citrate, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Highly purified recombinant protein corresponding to human Interleukin-10, expressed in E.coli
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human Interleukin-10
