Interleukin-10 (IL-10) is a cytokine whose main biological function is to suppress the immune response by induction of a signal(s) leading to inhibition of synthesis of a number of cytokines and their cellular receptors. Signal transduction is initiated upon formation of a ternary complex of IL-10 with two of its receptor chains, IL-10R1 and IL-10R2, expressed on the cell membrane. The affinity of IL-10R1 toward IL-10 is very high, which allowed determination of the crystal structure of IL-10 complexed with the extracellular/soluble domain of IL-10R1, while the affinity of IL-10R2 toward either IL-10 or IL-10/sIL-10R1 complex is quite low.

Suitable for use in Flow Cytometry. Other applications not tested.

Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Applications are based on unconjugated antibody.

Soluble human Interleukin 10 Receptor alpha.

Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.

Purified by affinity chromatography.

Recognizes the IL-10-binding epitope of human Interleukin 10 Receptor alpha. Species Crossreactivity: Cynomolgus, Rhesus